The chromatographic separation behavior was very robust between pH values 6 and 9, and the dynamic binding capacity was estimated to 60 µg/ml resin. The RNA fraction bound most strongly to the resin and could be eluted only at very high salt concentrations (2.0 M NaCl). Amount of medium The amount of Capto adhere medium needed can be calculated by: column cross sectional area (cm2) x bed height (cm) x compression factor (settled medium bed height/packed. Materials needed Capto adhere Glass filter funnel Plastic spoon Filtering flask Measuring cylinder 20 ethanol. Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein and endotoxin contaminations. Packing Capto adhere in a XK 16/20 column. Capto MMC is designed to increase speed and throughput in capture and intermediate purification. The most pronounced are ionic interaction, hydrogen bonding, and hydrophobic interaction. This property could be used to isolate plasmid DNA from a crude Escherichia coli extract. The Capto MMC ligand exhibits many functionalities for interaction with a target molecule. Capto Adhere proved to offer a very strong binding of nucleic acids. In multimodal chromatography, the ligand interacts with the target molecule through multiple types of interactions. HiTrap Capto MMC 1 ml, and HiTrap Capto adhere 1 ml. Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. Capto adhere is a multimodal anion exchanger. Multimodal ligands (MMC, adhere) provide ionic interaction, hydrogen bonding. The strong ion exchange multimodal ligand gives a different selectivity compared with traditional ion exchangers. Note that the Capto Core 700 ligand is found only in the core of the bead OH S NH O OHO O O -. Capto adhere Capto adhere is a multimodal BioProcess resin designed for post-protein A purification of monoclonal antibodies (mAbs) at process scale (Fig 1). The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. Capto MMC, Capto adhere, and Capto Core are shown as examples.

Multimodal chromatography is widely used for isolation of proteins because it often results in improved selectivity compared to conventional separation resins.

Capto adhere ligand